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KMID : 1177720160100010005
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Journal of Alternatives to Animal Experiments
2016 Volume.10 No. 1 p.5 ~ p.13
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Development of an In vitro Phototoxicity Test Using the Human Reconstituted Epidermis Model Developed in Korea
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Lee Yong-Sun
Yi Jung-Sun
Lim Hye-Rim
Kim Tae-Sung
Ahn Il-Young
Koh Kyoung-Yuk
Kim Joo-Hwan
Park Hye-Kyung
Lee Jong-Kwon
Sohn Soo-Jung
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Abstract
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Human reconstituted epidermis models have been widely used in evaluating phototoxicity. However, human reconstituted epidermis models made in Korea have not been employed to assess phototoxicity. In this study, we conducted an in vitro phototoxicity test with Neoderm®-E, human reconstituted epidermis model from Korea, in order to see if the model could be used for the evaluation of phototoxicity. Firstly, we performed an UVA sensitivity test and selected 6 J/cm2 for an UVA irradiation dose. Chlorpromazine as a positive control was then tested to confirm that the substance induces phototoxicity in this model. Afterwards, 3 phototoxins and 2 non-phototoxins were tested using Neoderm®-E and EpidermTM, a generally used phototoxicity test model, to compare prediction results for each chemical. The results indicated that all those 5 chemicals were correctly predicted with Neoderm®-E, which was accordant with the phototoxic information on them. However, only 3 chemicals were correctly predicted with EpidermTM. Consequently, the predictive capacity of the test with Neoderm®-E could be higher than that using EpidermTM. Overall, we reached the conclusion that Neoderm®-E could be used in evaluating phototoxicity.
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KEYWORD
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Human reconstituted epidermis model, phototoxicity, alternative test method, EpidermTM, Neoderm®-E
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